◯ Virus
African swine fever virus
The p72 gene of African swine fever virus (ASFV), the causative agent of African swine fever, was detected by ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® PCR Master Mix
○ Primers and Probes
- Front Vet Sci. 2022; 9: 926881.
○ Template
Synthetic dsDNA fragment (derived from the ASFV p72 gene region)
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | |
|---|---|---|---|
| HS | 98 | 5 | 1 |
| DN | 98 | 4 | 60 |
| AE | 65 | 12 |
(Time required: approx. 20.5 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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