◯ Virus
Bovine leukemia virus
The pol gene encoded in the provirus of bovine leukemia virus (BLV), the causative agent of bovine leukemia, was detected by ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® PCR Master Mix
○ Primers and Probes
PLoS Pathog. 2016;12(4):e1005588.
○ Template
Synthetic dsDNA fragment (derived from the BLV pol gene)
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | |
|---|---|---|---|
| HS | 98 | 5 | 1 |
| DN | 98 | 1.8 | 50 |
| AE | 70 | 3.6 |
(Time required: approx. 9 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
Related Products
