◯ Virus
Mpox virus (MPXV) (1)
Genomic DNA of MPXV (Monkeypox virus), the causative agent of mpox, was detected by ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® PCR Master Mix
○ Primers and Probes
Nihon Gene Research laboratories Inc.
Monkeypox qPCR Detection Primer/Probe
- Forward Primer : Hypercool_MPXV G2R_WA-Fwd
- Reverse Primer : Hypercool_MPXV G2R_WA-Rev
- Probe : Hypercool_MPXV G2R_WA-Probe
○ Template
MPXV Genomic DNA
○ PCR Protocol
| Temp.(°C) | Time (sec) | Cycle | |
|---|---|---|---|
| HS | 98 | 5 | 1 |
| DN | 98 | 1.8 | 50 |
| AE | 60 | 3.6 |
(Time required: approx. 9 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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