◯ Virus
Adenovirus
Genomic DNA of Adenovirus (AdV) was detected by ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® PCR Master Mix
○ Primers and Probes
Applied BiosystemsTM
- TaqManTM Microbe Assay Type 2
Assay ID: Vi99990001_po
Gene Symbol:Human mastadenovirus B and others
○ Template
Adenovirus genomic DNA
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | |
|---|---|---|---|
| HS | 98 | 5 | 1 |
| DN | 98 | 3 | 50 |
| AE | 65 | 8 |
(Time required: approx. 13 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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