◯ Bacteria
Escherichia coli O157 (EHEC) genomic
We detected the eae gene (intimin), which is essential for intestinal mucosal adherence of enterohemorrhagic Escherichia coli O157 (EHEC), using ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® PCR Master Mix
○ Primers and Probes
J Appl Microbiol. 2009 Feb;106(2):410-20.
○ Template
Genomic DNA of Escherichia coli O157:H7
○ 測定プロトコール 【所要時間：約10分】
| Temp.(°C) | Time(sec) | Cycle | |
|---|---|---|---|
| HS | 96 | 5 | 1 |
| DN | 96 | 3 | 50 |
| AE | 60 | 4 |
(Time required: approx. 10 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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