◯ Virus
Dengue virus type 1
Positive control RNA of Dengue virus type 1 (DENV-1) was detected by ultra-rapid real-time RT-PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini mini PCR chips
- GeneSoC® RT-PCR Master Mix
○ Primers and Probes
- Dengue virus Primer/Probe Mix (Takara Bio Inc., RC525A）
○ Template
- Dengue virus Positive Control RNA（Takara Bio Inc., RC625A）
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | |
|---|---|---|---|
| RT | 42 | 60 | 1 |
| HS | 96 | 10 | 1 |
| DN | 96 | 4 | 50 |
| AE | 54 | 4 |
(Time required: approx. 10.5 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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