◯ Bacteria
Macrolide-resistant Bordetella pertussis
A gene associated with macrolide resistance in Bordetella pertussis (23S rRNA mutation : A2047G) was detected by ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- CycleavePCRTM Reaction Mix (CY505A, Takara Bio Inc.)
○ Primers and Probes
Pathogen Detection Manual: Pertussis (Version 4.0) Source: National Institute for Health Crisis Management – Infectious Disease Information Website Section (3)-9: Detection of Macrolide-Resistant Strains Refer to the A2047G Cycleave PCR Method.
○ Template
An aqueous solution containing DNA fragments of the mutant (A2047G) and wild type (A2047).
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | |
|---|---|---|---|
| HS | 98 | 10 | 1 |
| DN | 98 | 3 | 50 |
| AE | 57 | 9 |
(Time required: approx. 13 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact our representative.
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