◯ Virus
Human cytomegalovirus
Positive control DNA of Human cytomegalovirus （HCMV） was detected by ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® PCR Master Mix
○ Primers and Probes
- Human cytomegalovirus Primer/Probe Mix
（Takara Bio Inc., RC527A）
○ Template
- Human cytomegalovirus Positive Control DNA
（Takara Bio Inc., RC627A）
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | HS | 96 | 10 | 1 |
|---|---|---|---|
| DN | 96 | 4 | 50 |
| AE | 58 | 10 |
(Time required: approx. 14 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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