◯ Bacteria
Clostridioides difficile
Genomic DNA of Clostridioides difficile (C. difficile) was detected by ultra-rapid real-time PCR.
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® PCR Master Mix
○ Primers and Probes
- Clostridioides difficile Primer/Probe Mix
（Takara Bio Inc., RC706A）
○ Template
- Clostridioides difficile Positive Control DNA
（Takara Bio Inc., RC756A）
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | HS | 96 | 10 | 1 |
|---|---|---|---|
| DN | 96 | 4 | 50 |
| AE | 56 | 4 |
(Time required: approx. 9 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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