◯ Virus
swine fever virus
The 5’-UTR region of Classical swine fever virus (CSFV), the causative agent of Classical swine fever, was detected by ultra-rapid real-time RT-PCR
○ Instruments and reagents
- GeneSoC® mini R
- GeneSoC® mini PCR chips
- GeneSoC® RT-PCR Master Mix
○ Primers and Probes
PLoS One. 2013;8(7):e71019.
○ Template
Synthetic RNA (derived from the CSFV The 5’-UTR region)
○ PCR Protocol
| Temp.(°C) | Time(sec) | Cycle | |
|---|---|---|---|
| RT | 42 | 60 | 1 |
| HS | 98 | 5 | |
| DN | 98 | 1.8 | 50 |
| AE | 65 | 3.6 |
(Time required: approx. 10 minutes)
- HS：Hot Start
- DN：Denature
- AE：Annealing / Extension
※For more detailed experimental conditions, please contact us.
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